Protein_Domain

Part:BBa_K165007:Design

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-26)

Gli-1 DNA-binding domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[http://2010.igem.org/Team:Slovenia Team Slovenia 2010] deposited a modified variant of Gli-1 intended for easier isolation, purification and other experiments. We added HIS tag to N-terminal side of Gli1 sequence and N-terminal split CFP on C-terminal part of Gli1 after an extension linker. We deposited modified variant of Gli1 as Gli1_link_nCFP BBa_K323016 and additionally characterized it with several methods.

Source

-

References

Kasper M., Regl G., Frischauf A., Aberger F. 2006. GLI transcription factors: Mediators of oncogenic Hedgehog signalling. EUROPEAN JOURNAL OF CANCER 42 (2006) 437–445